Recently there’s been a lot of excitement building up within the molecular biology community about sequencing long reads, and by “long reads” I mean reads of several kb in length…
COFFEE BREAK NEWS FOR LIFE SCIENTISTS
The method of choice for nucleic acid (DNA, RNA) quantification in all areas of molecular biology is real-time PCR or quantitative PCR (qPCR).
Will ddPCR replace qPCR technology?
Amplification efficiency higher than 100% would mean that in each qPCR cycle, more than two copies of the target sequence have been generated.
Is this possible?
How to present the ratio data?
Which are the most probable reasons for a false positive result and how to address the issue?
Researchers often wonder which statistical tests they should use with their qPCR data. Most frequently these questions arise when analysing gene expression data.
The scandal has led to the human community in the EU benefiting from the better labeling of foods!
Concise overview over the current situation.