COFFEE BREAK NEWS FOR LIFE SCIENTISTS

DNA qPCR quantification

qPCR Data Analysis Made Easy

Despite the advancement of new molecular biology techniques like ddPCR or  RNAseq, quantitative PCR (qPCR) is still a golden standard for the quantification of nucleic acids, with applications in diagnostics and research. The method is based on real-time monitoring of amplification of the target DNA allowing its quantification (see basics). Yet, for the method to be quantitative, it has to be done properly.

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