Since we live in the digital era, why shouldn’t a popular method like PCR go digital as well? What is digital PCR (dPCR) and how it is different in comparison to PCR and qPCR, has been explained in the webinar “Why go Digital in qPCR”.
Price is often one of the most important factors when considering a purchase of a new qPCR thermal cycler. One reason for this is that it obviously has a direct and immediate impact on your budget.
Can we trust your published qPCR data?
The pressure to publish is causing shortcuts which result in misleading papers being published.
The method of choice for nucleic acid (DNA, RNA) quantification in all areas of molecular biology is real-time PCR or quantitative PCR (qPCR).
Will ddPCR replace qPCR technology?
Amplification efficiency higher than 100% would mean that in each qPCR cycle, more than two copies of the target sequence have been generated.
Is this possible?
How to present the ratio data?
Which are the most probable reasons for a false positive result and how to address the issue?
Researchers often wonder which statistical tests they should use with their qPCR data. Most frequently these questions arise when analysing gene expression data.