Despite the advancement of new molecular biology techniques like ddPCR or RNAseq, quantitative PCR (qPCR) is still a golden standard for the quantification of nucleic acids, with applications in diagnostics and research. The method is based on real-time monitoring of amplification of the target DNA allowing its quantification (see basics). Yet, for the method to be quantitative, it has to be done properly.
Relative Quantification Data Presentation
How to present the ratio data?
DNA Contamination of PCR Reagents – a Great Risk for False Positive Signals
Which are the most probable reasons for a false positive result and how to address the issue?
Parametric or Non-Parametric Tests for qPCR Data?
Researchers often wonder which statistical tests they should use with their qPCR data. Most frequently these questions arise when analysing gene expression data.