Extraction of DNA before its amplification is an essential step for the measurement of any DNA target. It releases the DNA and removes substances inhibitory to PCR that are initially present in the matrix.
Since we live in the digital era, why shouldn’t a popular method like PCR go digital as well? What is digital PCR (dPCR) and how it is different in comparison to PCR and qPCR, has been explained in the webinar “Why go Digital in qPCR”.
Will ddPCR replace qPCR technology?